氧化平衡评分与血管内皮功能指标的横断面关联研究

Aü cross-sectional association study on oxidative balance score and vascular endothelial function indicators

摘要:

目的]分析氧化平衡评分(OBS)对血管内皮功能的影响,为制定针对性干预措施、预防血管内皮功能障碍提供实证依据。 [方法]本研究选取北京市顺义区汉族常住居民为研究对象,采用文献资料法、问卷调查法和高效液相色谱-串联质谱法测算研究人群的OBS；采用Vivid-7彩色多普勒超声、UNEX EF血管内皮功能检测技术和ELISA评估受试者血管内皮形态结构和功能状态；运用独立样本t检验、单因素方差分析、卡方检验及多因素Logistic回归分析,探究OBS与血管内皮功能指标间的关系。 [结果]随着OBS水平的升高,8-异前列腺素F2α(FIP)含量降低,OBS2组和OBS3组FIP水平显著低于OBS1组(P＜0.01)；OBS2组和OBS3组发生高FIP表达的风险显著降低(P＜0.01)。高FIP组血流介导的血管舒张功能(FMD)和血清一氧化氮(NO)/内皮素1(ET-1)比值显著低于低FIP组(P＜0.05)；高FIP组的颈动脉内膜中膜厚度(CIMT)和血清ET-1水平显著高于低FIP组(P＜0.01)；低FIP组发生血管内皮功能减退的风险显著低于高FIP组(P＜0.05)。OBS3组的ET-1和内皮型一氧化氮合酶(eNOS)水平显著低于OBS1组和OBS2组(P＜0.01),OBS3组的NO/ET-1比值显著高于OBS1组和OBS2组(P＜0.01)；随着OBS值的升高,CIMT逐步减小,OBS3组CIMT显著低于OBS1组(P＜0.01),CIMT异常增厚(CIMT＞1.00 mm)发生率显著下降(P＜0.01)；FMD随着OBS值的升高呈上升趋势,OBS3组FMD显著高于OBS1组(P＜0.05),FMD异常降低(FMD＜6%)发生率亦显著降低(P＜0.01)。 [结论]OBS升高可有效降低机体氧化应激水平,显著改善血管内皮形态结构和生理功能。

Abstract:

Aim To analyze the effect of the oxidative balance score (OBS) on vascular endothelial function and to provide empirical evidence for developing targeted interventions and preventing vascular endothelial dysfunction. Methods This study selected permanent Han residents of Shunyi District, Beijing as the research subjects. OBS of the study population was calculated using literature review, questionnaire survey, and high-performance liquid chromatography-tandem mass spectrometry. Vascular endothelial morphology and functional status were assessed using Vivid-7 color Doppler ultrasound, UNEX EF vascular endothelial function testing technology, and ELISA. Independent samples t-test, one-way analysis of variance, chi-square test, and multivariate Logistic regression analysis were used to explore the relationship between OBS and vascular endothelial function indicators. Results As OBS level increased, the content of 8-iso-prostaglandin F2α (FIP) decreased. FIP levels in OBS group 2 and 3 were significantly lower than those in OBS group 1 (P＜0.01). The risk of high FIP expression was significantly reduced in OBS group 2 and 3 (P＜0.01). Flow-mediated dilation (FMD) and serum nitric oxide/endothelin-1 (NO/ET-1) ratio were significantly lower in the high-FIP group than in the low-FIP group (P＜0.05). Carotid intima-media thickness (CIMT) and serum ET-1 levels were significantly higher in the high-FIP group than in the low-FIP group (P＜0.01). The risk of vascular endothelial dysfunction was significantly lower in the low-FIP group than in the high-FIP group (P＜0.05). ET-1 and endothelial nitric oxide synthase (eNOS) levels in OBS group 3 were significantly lower than those in groups 1 and 2 (P＜0.01), while the NO/ET-1 ratio in OBS group 3 was significantly higher than that in groups 1 and 2 (P＜0.01). CIMT progressively decreased with increasing OBS values, CIMT in OBS group 3 was significantly lower than that in the OBS group 1 (P＜0.01), and the incidence of abnormally thickened CIMT (CIMT >1.00 mm) was significantly reduced (P＜0.01). FMD showed an increasing trend with rising OBS value, FMD in OBS group 3 was significantly higher than that in OBS group 1 (P＜0.05), and the incidence of abnormally reduced FMD (FMD＜6%) was also significantly reduced (P＜0.01). Conclusion Elevated OBS can effectively reduce systemic oxidative stress levels and significantly improve vascular endothelial morphology and physiological function.