阿司匹林对血管平滑肌细胞增殖及增殖细胞核抗原表达的影响

Effects of Aspirin on the Proliferation of Vascular Smooth Muscle Cells and the Expression of Proliferating Cell Nuclear Antigen

摘要:

目的探讨阿司匹林对大鼠血管平滑肌细胞增殖的影响及相关机制。方法体外培养大鼠主动脉平滑肌细胞,用不同浓度的阿司匹林处理,并且设置对照组,采用MTT法检测阿司匹林对血管平滑肌细胞增殖活性的影响,用免疫细胞化学和逆转录聚合酶链反应检测阿司匹林作用后血管平滑肌细胞中增殖细胞核抗原的表达。结果较高浓度(5×10-3mol/L)的阿司匹林对血管平滑肌细胞的增殖有明显抑制作用(p<0.05),相对抑制率为31.5%。与对照组相比,该浓度组血管平滑肌细胞中增殖细胞核抗原的蛋白和mRNA表达量均显著降低(p<0.05)。结论阿司匹林可能通过降低增殖细胞核抗原的表达来抑制血管平滑肌的增殖,对心血管系统具有抗血小板聚集之外的保护作用。

关键词:病理学与病理生理学;阿司匹林;血管平滑肌细胞;细胞增殖;增殖细胞核抗原;免疫细胞化学;基因表达

Abstract:

Aim To elucidate effects of aspirin on the proliferation of vascular smooth muscle cells (VSMC) and the mechanism involved. Methods The rat aortic smooth muscle cells were treated with various concentrations of aspirin for 24 hours, then MTT assay was performed to determine the number of the cells. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunocytochemistry. In addition, reverse transcription polymerase chain reaction (RT-PCR) was applied to detect the mRNA expression of PCNA. Results High concentration (5×10-3 mol/L) of aspirin inhibited the proliferation of VSMC and the relative inhibition ratio was 31.5%. Compared with the control group, the expression of PCNA protein and mRNA in the 5×10-3 mol/L aspirin group were significantly decreased (312±48 and 0.663±0.035, p<0.05). Conclusion Aspirin may arrest the proliferation of VSMC by inhibiting the expression of PCNA.

Key words:Aspirin; Vascular Smooth Muscle Cell; Cell Proliferation; Proliferating Cell Nuclear Antigen; Immunocytochemistry; Gene Expression