碱性成纤维细胞生长因子对血管性痴呆大鼠海马区脑血管生成的影响

Effect of Basic Fibroblast Growth Factor on Cerebral Vascular in Hippocampus of Vascular Dementia Rats

摘要:

目的皮下注射碱性成纤维细胞生长因子于血管性痴呆大鼠,研究用药前后对大鼠海马区脑血管生成的影响。方法制作血管性痴呆大鼠模型,随机取用血管性痴呆大鼠模型12只,分碱性成纤维细胞生长因子处理组6只,生理盐水对照组6只。另外,取假手术组6只。皮下注射碱性成纤维细胞生长因子于血管性痴呆大鼠。治疗5周后,以Morris水迷宫定位航行试验和空间探索试验来检测大鼠的学习记忆能力,检测大鼠血清血管内皮生长因子变化,第Ⅷ因子相关抗原多克隆抗体免疫组织化学染色,观察海马阳性细胞数的变化。结果碱性成纤维细胞生长因子治疗5周后,假手术组、生理盐水对照组、碱性成纤维细胞生长因子处理组的平台象限滞留时间分别为14.3±3.1 s、7.4±2.9 s和12.6±2.7 s。假手术组和碱性成纤维细胞生长因子处理组的平台期保持时间要明显长于生理盐水对照组(P<0.05),显示碱性成纤维细胞生长因子处理组空间记忆能力较生理盐水对照组明显提高。假手术组、生理盐水对照组、碱性成纤维细胞生长因子处理组的血管内皮生长因子含量分别是8.14±1.53、6.07±0.18、9.19±0.29,碱性成纤维细胞生长因子处理组与生理盐水对照组比较有统计学意义(P<0.05)。假手术组、碱性成纤维细胞生长因子处理组、生理盐水对照组海马CA1区F8免疫反应阳性神经元计数分别为105.38+1.45、101.32+1.28、43.03+2.11,碱性成纤维细胞生长因子处理组与生理盐水对照组组间差异有显著性(P<0.01)。结论皮下注射碱性成纤维细胞生长因子后能刺激海马区脑血管生成,改善大鼠的学习记忆能力。

关键词:碱性成纤维细胞生长因子;血管性痴呆;大鼠;脑血管;血管内皮生长因子;第Ⅷ因子相关抗原多克隆抗体

Abstract:

Aim To study the change of cerebral vascular in hippocampus of vascular dementia rats after hypodermic injection of basic fibroblast growth factor. Methods After the vascular dementia model was reproduced,the 12 model rats were randomly divided into basic fibroblast growth factor(bFGF) treatment group,normal saline treatment group.The 6 rats were in sham group.The vascular dementia rats in therapy group were treated by hypodermic injection of bFGF.After 5 weeks,abilities of learning and memory of three section rats were tested by using the Morris water maze.The changes of vascular endothelial growth factor(VEGF) in rats serum were tested.Immunochemistry staining of factor Ⅷ-related antigen(F8) was used to observe the number of nestin positive neurons in hippocampus. Results The time spent on the platform was 14.3±3.1 s,7.4±2.9 s and 12.6±2.7 s in the sham,bFGF treatment group and normal saline treatment group.The VEGF in ratsserum was 8.14±1.53,6.07±0.18,9.19±0.29.It was more significantly increased in bFGF treatment group than that in normal saline treatment group(P<0.05).The number of the F8 positive neurons in the hippocampus were 105.38+1.45,101.32+1.28,43.03+2.11.It was more significantly increased in bFGF treatment group than that in normal saline treatment group. Conclusions bFGF by hypodermic injection can migrate to hippocampus area and induce hippocampus to create cerebral vascular.bFGF can increase abilities of learning and memory of rats significantly.

Key words:Basic Fibroblast Growth Factor;Vascular Dementia;Rat;Cerebral Vascular;Vascular Endothelial Growth Factor;Factor Ⅷ-related Antigen