99mTc标记抗心肌肌钙蛋白T单抗在急性心肌损伤大鼠模型中的生物学分布

The Biological Distribution of 99mTc-anti-cardiac Troponin T Monoclonal Antibody in the Rats with Acute Myocardial Damage

摘要:

目的研究99mTc标记抗心肌肌钙蛋白T单抗在急性心肌损伤大鼠模型中的生物学分布。方法先制备99mTc标记抗心肌肌钙蛋白T单抗和建立急性心肌损伤大鼠模型。本研究分3组：实验组、对照组和空白组，各组20只大鼠。实验组急性心肌损伤大鼠静脉注射99mTc标记抗心肌肌钙蛋白T单抗0.3 mCi，分别于注射后2、4、8、12 h时处死（每时间点5只），取血液、肝、脾、肾、肌肉、结肠、肺、心脏，以计算每克组织放射性计数占总注入计数的百分比（ID%/g）及心/肺比值。对照组（急性心肌损伤大鼠）和空白组（正常心肌大鼠）分别静脉注射99mTc-N-IgG和99mTc标记抗心肌肌钙蛋白T单抗0.3 mCi，其它处理方法同实验组。结果实验组的ID%/g和心/肺比值均较对照组和空白组显著为高，提示急性损伤心肌对99mTc标记抗心肌肌钙蛋白T单抗是特异性摄取，且摄取高峰时间为4 h。结论99mTc标记抗心肌肌钙蛋白T单抗有望作为亲心肌损伤显像剂用于诊断急性心肌损伤。

关键词:心肌肌钙蛋白T; 急性心肌损伤; 放射免疫显像; 亲心肌梗塞显像

Abstract:

AimTo investigate the biological distribution of 99mTc-anti-cardiac troponin T monoclonal antibody (99mTc-AcTnTMA) in the rats with acute myocardial damage.MethodsPrepared 99mTc-AcTnTMA and built the rat model of acute cardiac muscle damage.Then 60 rats were randomized into the experimental group, the control group and the blank group.There are twenty rats in one group.In the experimental group, 20 rats with acute myocardial damage were injected with 0.3 mCi 99mTc-AcTnTMA and were killed at 2, 4, 8 and 12 h after injection respectively (5 rats).Blood, liver, spleen, kidney, muscle, colon, lung and heart of each rat were taken and the injected dosage (ID%/g) and the ratio of ID%/g for heart-to-lung ratio (HLR) was calculated.In the control group and the blank group, 20 rats with acute myocardial damage were injected with 0.3 mCi 99mTc-N-IgG and 99mTc-AcTnTMA respectively and were killed and followed subsequent procedure in the same way as the experimental group. ResultsThe value of ID%/g and HLR in the experimental group were significantly higher than the other groups, which hinted that acute injury myocardium could uptake specifically the 99mTc-AcTnTMA and the specific uptake was observed with its peak 4 h after injection.Conclusion99mTc-AcTnTMA could be a useful tracer of myocardial imaging to diagnose acute myocardial damage.

Key words:Myocardium Calcium Protein T;Acute Myocardial Damage;Radioimmunoimaging;Infarct Avid Imaging