基于FAK-F-actin通路探讨岩藻糖基转移酶8对泡沫细胞运动能力的影响

Effect of fucosyltransferase 8 on the migration of foam cell based on FAK-F-actin signaling pathway

摘要:

目的 探讨岩藻糖基转移酶8(Fut8)表达下调对泡沫细胞运动能力的影响及机制。方法 建立泡沫细胞模型,检测Fut8表达的改变,并明确其与泡沫细胞运动能力之间的直接联系。检测Fut8过表达对细胞内纤维型肌动蛋白(F-actin)形成的影响及其上游分子局部黏着斑激酶(FAK)的活化情况。结果 泡沫细胞形成过程中,Fut8的表达明显下调,而过表达Fut8能修复泡沫细胞受损的运动能力。F-actin的表达随着泡沫细胞运动能力下降而明显下调,且受到Fut8的调节。FAK的磷酸化在Fut8表达下调时受到明显抑制,过表达Fut8能导致FAK的磷酸化明显增强。结论 Fut8-FAK-F-actin通路受到抑制可能是泡沫细胞运动能力减弱的原因之一。

Abstract:

Aim To investigate the effect and mechanism of fucosyltransferase 8 (Fut8) on the migration of foam cells. Methods The change of Fut8 expression was detected after the foam cell model was established, and the direct relationship between foam cell motility and Fut8 expression was identified. The effects of Fut8 overexpression on the fibrous actin (F-actin) polymerization and the phosphorylation of upstream molecule focal adhesion kinase (FAK) were examined. Results During the formation of foam cells, the expression of Fut8 was significantly down-regulated, and overexpression of Fut8 could repair the impaired migration of foam cells. Expression of F-actin was significantly down-regulated as foam cell motility decreased, and was regulated by Fut8. Phosphorylation of FAK was significantly inhibited when Fut8 expression was down-regulated, and overexpression of Fut8 could result in significantly enhanced phosphory-lation of FAK. Conclusion Inhibition of Fut8-FAK-F-actin pathway may be one of the reasons for the weakening of foam cell motility.