超声处理的聚苯乙烯纳米塑料对巨噬细胞脂质蓄积的影响

Effect of ultrasound treated polystyrene nanoplastics on lipid accumulation in macrophages

摘要:

目的]探究超声处理的聚苯乙烯纳米塑料(PS-NP)对巨噬细胞源性泡沫细胞脂质蓄积的影响。 [方法]采用CCK-8法检测PS-NP和高频超声处理的PS-NP(UPS-NP)对巨噬细胞活力的影响,采用油红O染色试剂盒和胆固醇检测试剂盒检测细胞内脂质蓄积情况,采用RT-qPCR和Western blot检测胆固醇摄取和流出相关mRNA和蛋白表达以及细胞胞葬相关受体mRNA水平的变化。 [结果]UPS-NP对巨噬细胞活力无显著影响,但UPS-NP可以显著增加巨噬细胞源性泡沫细胞形成,增加泡沫细胞脂质蓄积。UPS-NP可显著上调CD36、清道夫受体A1(SR-A1)的mRNA和蛋白表达,但不影响胆固醇外排相关受体ATP结合盒转运体A1(ABCA1)、清道夫受体B1(SR-B1)的mRNA和蛋白表达。UPS-NP不影响与细胞胞葬相关受体mRNA水平。 [结论]超声处理后的PS-NP可以显著增加巨噬细胞源性泡沫细胞脂质蓄积,其机制与上调CD36和SR-A1表达有关。

Abstract:

Aim To explore the effect of ultrasound-treated polystyrene nanoplastics (PS-NP) on lipid accumulation in macrophage-derived foam cells. Methods The CCK-8 method was used to detect the effects of PS-NP and high-frequency ultrasound-treated PS-NP (UPS-NP) on the activity of macrophages, oil red O staining and cholesterol detection kit were used to detect the intracellular lipid accumulation, RT-qPCR and Western blot were used to detect the expression of mRNA and protein related to cholesterol uptake and efflux, as well as mRNA levels of cell burial related receptors. Results UPS-NP had no significant effect on the activity of macrophages, but UPS-NP could significantly increase the formation of macrophage-derived foam cells and increase the lipid accumulation in foam cells. UPS-NP could significantly upregulate the mRNA and protein expression of CD36 and scavenger receptor-A1 (SR-A1), but did not affect the mRNA and protein expression of cholesterol efflux related receptors ATP-binding cassette transporter A1 (ABCA1) and scavenger receptor-B1 (SR-B1). UPS-NP did not affect the mRNA levels of receptors related to cell burial processes. Conclusion Ultrasound-treated PS-NP can significantly increase lipid accumulation in macrophage-derived foam cells, and its mechanism is related to the upregulation of CD36 and SR-A1 expression.